Figure 7. Function of β-cells cultured in various conditions.
(a) qPCR analysis of β-cell specific gene (MafA, Ins2 and Pdx1) expression in cells cultured with various types of PEG-co-PLL microbeads at 21 days. (b) Glucose stimulated insulin secretion (GSIS) of β-cells cultured with Beads + EphA/EphrinA + DCM or in 2D Non-coating (TCPS) surface. Cells were cultured in a 48-well microplate, with approximately 1.2 × 105 cells/well. In the microbead containing conditions, 3.6 × 105 microbeads/well were used. n = 4. The error bars represent the s.d. of three measurements.