Figure 1.

SdBP associated with Sd and antagonized Sd-Yki-induced overgrowth. (A) Co-immunoprecipation of HA-Sd and 3× Flag-SdBP in S2 cells. The indicated constructs were transfected into S2 cells, followed by immunoprecipitation and western blot analysis with the indicated antibodies. The arrowhead indicates IgG chain. (B) Luciferase activity of a luciferase reporter plasmid with 3× Sd-binding sites and the control vector in S2 cells cotransfected with the indicated constructs. Data were presented as mean ± s.d. (n = 3). (C-F) Gain of function of SdBP induced growth defects and also reduced GMR-Yki-induced overgrowth. Adult eyes expressing GMR-Gal4 (C), GMR-Gal4/UAS-SdBP (D), GMR-Gal4/UAS-Yki (E), and GMR-Gal4/UAS-(SdBP+Yki) (F). (C'-F') BrdU staining of wild-type eye discs (C'), and eye discs expressing UAS-SdBP (D'), UAS-Yki (E') or UAS-(Yki+SdBP) (F') under the control of GMR-Gal4.