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. 2013 Sep 3;23(10):1201–1214. doi: 10.1038/cr.2013.120

Figure 5.

Figure 5

SdBP formed a complex with Sd-Yki. (A) S2 cells expressing the indicated proteins were collected for the two-step immunoprecipitation and analyzed by western blot. Cell lysates were first immunoprecipitated with M2-Flag beads, and then eluted for a second immunoprecipitation with Myc antibody. The arrowheads indicate IgG chain. (B-E''') Confocal images of third-instar wing discs expressing Myc-Yki (B-B'), or Myc-Yki with HA-Sd (C-C') or Myc-Yki with SdBP-GFP (D-D''') or Myc-Yki with HA-Sd and SdBP-GFP (E-E'''). Discs were stained with α-Myc (red), α-HA (magenta) antibodies and DAPI (blue). (F-G') Adult eyes expressing GMR-Gal4/UAS-(Sd+Yki) (F) and GMR-Gal4/UAS-(Sd+Yki+SdBP) (G). BrdU staining of eye discs expressing UAS-(Sd+Yki) (F') and UAS-(Sd+Yki+SdBP) (G') under the control of GMR-Gal4.