Figure 2.
The (HhH)2 domain of FAAP24 is required for FANCM interaction and DNA binding. (A) Schematics of FAAP24 truncation mutants used for Co-IP experiment in B. Pink-shaded areas depict the degenerated nuclease motif. Green-shaded areas depict the tandem HhH repeat. (B) Co-IP of Myc-tagged wild-type FAAP24 or the truncation mutants with FANCM. HEK293T cells were co-transfected with plasmids encoding Myc-tagged FAAP24 and FANCM, and the cell lysates were subjected to immunoprecipitation with an Myc antibody. Asterisks indicate nonspecific bands associated with anti-Myc antibody. (C) SPR assay measuring ssDNA-binding affinity of wild-type FAAP24 and the C-terminal (HhH)2 domain truncation mutant (ΔC).