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Co-IP of Myc-tagged wild-type or mutant FAAP24 with FANCM. (A-B) Lysates of FAAP24−/− cells stably complemented with Myc-tagged wild-type or mutant FAAP24 were subjected to immunoprecipitation with an Myc antibody, and western blot analysis was performed to detect FANCM and FAAP24. Arrows indicate Myc-tagged FAAP24 proteins. The # marks Myc-tagged D5 mutant. Asterisks indicate either nonspecific bands associated with the anti-Myc antibody or potential degradation bands.
