Table I. List of identified phosphoproteins and phosphopeptides from T. thermophilus HB27.
| Locus name/gene name | Protein description | Phosphopeptide | Phosphosites |
|---|---|---|---|
| TTC1279/metK | S-adenosylmethionine synthetase | TVVVSAQHpSPEVEQEQLREDLIR | pS238 |
| p[TVVVSAQHS]PEVEQEQLR | p[T230, S234, S238] | ||
| TTC0088/aroA | 3-phosphoshikimate 1-carboxyvinyltransferase | LRVPGDKpSVTHR | pS23 |
| FKEPEDVLDCGNAGpTLMR | pT94 | ||
| VPGDKp[SVT]HR | p[S23, T25] | ||
| TTC0089/cmk | Cytidylate kinase | GIVTIDGPpSASGK | pS11 |
| GIVTIDGPp[SASGKSS]VAR | p[S11, S13, S16, S17] | ||
| TTC0117 | Threonine synthase | LTEAFPVALVNpSVNPHRLEGQK | pS155 |
| TTC0159 | Riboflavin kinase/FMN adenylyltransferase | GVANVGpTRPTLGGGER | pT230 |
| TTC0166/ask | Aspartate kinase | YGGpT[S]VGDLER | pT11, p[S12] |
| TTC0169/sucD | Succinyl-CoA synthetase alpha chain | KPVVGFIGGRpSAPK | pS237 |
| TTC0291 | Phosphoglucomutase/phosphomannomutase | AAGGAMLTApSHNPPQYLGVK | pS102 |
| HLKAAGGAMLpTAp[S]HNPPQYLGVK | pT100, p[S102] | ||
| TTC0368 | Hypothetical protein TTC0368 | EEPTPpSRPR | pS219 |
| RLPVEVEAAEAp[SPPASPGPPREEPT]PpSRPR | pS219, p[S204, S208, T217] | ||
| RLPVEVEAAEASPPApSPGPPREEPp[TPS]RPR | pS208, p[T217, S219] | ||
| RLPVEVEAAEASPPASPGPPREEPpTPp[S]RPR | pT217, p[S219] | ||
| TTC0408/oah1 | O-acetyl-l-(homo)serine sulfhydrylase | FETLQLHAGYEPEPp[TTLS]R | p[T17, T18, S20] |
| TTC0553 | Ornithine aminotransferase | Fp[TGLDRVESHAEGPY]VWDTQGKR | p[T24, S31, Y37] |
| TTC0691/glmM | Phosphoglucosamine mutase/phosphoacetylglucosamine | ATAGAVIpSAp[S]HNPYQDNGIK | pS99, p[S101] |
| Mutase/phosphomannomutase | ATAGAVISApSHNPYQDNGIK | pS101 | |
| Ap[TAGAVISASHNPY]QDNGIKFFGPTGEKLPDEAEEEIER | p[T93, S99, S101, Y105] | ||
| Ap[TAGAVISASHNPY]QDNGIK | p[T93, S99, S101, Y105] | ||
| TTC0731/thyX | FAD-dependent thymidylate synthase | TASVNEIpSGR | pS92 |
| TTC0777 | Two-component response regulator | IREEAGPPVLAVpSAp[SVS]R | pS78, p[S80, S82] |
| EEAGPPVLAVSApSVSR | pS80 | ||
| IREEAGPPVLAVSAp[S]VpSR | pS82, p[S80] | ||
| TTC0800/trpC | Indole-3-glycerol phosphate synthase | GFGGVLVAEpSGYAR | pS215 |
| KQp[S]PpSAGVIR | pS58, p[S56] | ||
| TTC0805/valS | Valyl-tRNA synthetase | pSKGNVIDPLEMVER | pS532 |
| TTC0927 | Putative ribosomal associated protein | RHpSp[Y]QGPPPPEVR | pS105, p[Y106] |
| RHp[SY]QGPPPPEVR | p[S105, Y106] | ||
| TTC0992 | Hypothetical protein TTC0992 | FpTKDLDLWVDAEEAPRVLAAIR | pT36 |
| TTC1012/hicd | Homoisocitrate dehydrogenase | ILSCHATLFGAAp[T]pSPp[T]R | pS72, p[T71, T74] |
| TTC1029 | Hypothetical protein TTC1029 | pTKp[T]LEEFGHLAALLDGSR | pT180, p[T182] |
| KTKpTLEEFGHLAALLDGSR | pT182 | ||
| TTC1063 | Phosphoglucomutase/phosphomannomutase | EAAGLYLTGpSR | pS99 |
| AKEAAGLp[YLTGS]R | p[Y95, T97, S99] | ||
| EAAGLp[YLTGS]RRPFAFQGVK | p[Y95, T97, S99] | ||
| TTC1102/pyrG | CTP synthetase | RGEp[YLSQT]VQVIPHITDEIKER | p[Y117, S119, T121] |
| RGEp[YLSQTVQVIPHIT]DEIKER | p[Y117, S119, T121, T129] | ||
| TTC1136 | Phosphoenolpyruvate synthase | GGRp[TS]HAAIVAR | p[T419, S420] |
| GGRpTp[S]HAAIVAR | pT419, p[S420] | ||
| TTC1149 | Putative adenylosuccinate lyase protein | TEVLEAQEPFHEGQp[TGSSS]MPHKK | p[T268, S270, S271, S272] |
| TEVLEAQEPFHEGQp[TGSSS]MPHKKNPVGLENLTGVAR | p[T268, S270, S271, S272] | ||
| p[TEVLEAQEPFHEGQTGSSS]MPHKK | p[T254, T268, S270, S271, S272] | ||
| TEVLEAQEPFHEGQp[TGSSSMPHKKNPVGLENLT]GVAR | p[T268, S270, S271, S272, T286] | ||
| TTC1330/tuf | Translation elongation factor tu | GVpSREEVER | pS295 |
| TTC1351 | Hypothetical protein TTC1351 | HLEGNpSHAHLK | pS81 |
| TTC1381/pyrF | Orotidine 5′-phosphate decarboxylase | RGDIGpSp[T]AEAYAR | pS92, p[T93] |
| TTC1414 | Fructose-bisphosphate aldolase | INTDpTDLR | pT254 |
| TTC1420 | Metal dependent phosphohydrolase | NKVHpYLK | pY162 |
| TTC1488 | ABC transporter ATP-binding protein | pSELEIR | pS2 |
| TTC1541 | Acetylglutamate kinase | LLLVHGGpSAEp[T]NKVAEALGHPPR | pS38, p[T41] |
| TTC1600 | Inorganic pyrophosphatase | LDHIQDIGDVPEGVKQEIQHFFEp[TY]KALEAK | p[T139, Y140] |
| LDHIQDIGDVPEGVKQEIQHFFEp[T]pYKALEAK | pT139, p[Y140] | ||
| TTC1601 | Hypothetical protein TTC1601 | APVWEEEPPAKpTPp[SVELAPEEPPPAPT]PTPPPPAQGTR | pT127, p[S129, T142] |
| KApTAAPRPEPPKPPAQEAK | pT99 | ||
| TTC1611 | Pyruvate kinase | LNFpSHGAPEDHRR | pS39 |
| AIVVFp[TATGGS]AR | p[T380, T382, S385] | ||
| AIVVFp[T]ApTGGSAR | pT382, p[T380] | ||
| TTC1619 | Phosphoribosylaminoimidazole synthetase | EGDALLALPSSGPHp[TNGYS]LIR | p[T187, Y190, S191] |
| TTC1622/pilF | ATP-binding motif-containing protein PilF | RGGGRLEDTLVQpSGK | pS372 |
| RGGGRLEDpTLVQSGK | pT368 | ||
| TTC1630 | Phosphoglucomutase | ADGVLLTPpSHNPPEDGGFK | pS130 |
| ADGVLLpTPp[S]HNPPEDGGFK | pT128, p[S130] | ||
| TTC1659 | Phosphopentomutase | MREVNPGKDpTp[T]TGHWEFVGIHLEKPFR | pT80, p[T81] |
| EVNPGKDp[TT]pTGHWEFVGIHLEKPFR | pT82, p[T80, T81] | ||
| TTC1709/pckA | Phosphoenolpyruvate carboxylase | EGDVAVFFGLpSGp[T]GK | pS234, p[T236] |
| TTC1714/groEL | Chaperonin GroEL | ESTPApSAGAGDMDF | pS535 |
| TTC1772 | Kinase | EAVAPFGDpSLLVVGAEGYVK | pS249 |
| TTC1776/rplL | 50S ribosomal protein L7/L12 | IKEELSQApTVLELK | pT16 |
| TTC1798/ndk | Nucleoside diphosphate kinase | IpSQELAERHp[Y]AEHR | pS41, p[Y49] |
| ISQELAERHpYAEHR | pY49 | ||
| TTC1827 | Hypothetical protein TTC 1827 | KGELKApTAVPQGPLK | pT106 |
| TTC1852 | Peptide chain release factor 2 | LpYELERKK | pY297 |
| TTC1855 | Hypothetical protein TTC1855 | LVpSKSEVVR | pS36 |
| LVSKpSEVVR | pS38 | ||
| TTC1856 | Serine/threonine protein kinase | VMDFGLAYLLQEpSR | pS159 |
| HLpTRp[TGYT]LGTPTYMAPEQAK | pT163, p[T165, Y167, T168] | ||
| pTGYTLGTPTYMAPEQAK | pT165 | ||
| HLp[TRTGYTLGT]PTYMAPEQAK | p[T163, T165, YI67, T168, T171] | ||
| HLp[TRTGYTLGTPTY]MAPEQAK | p[T163, T165, Y167, T168, T171, T173, Y174] | ||
| HLpTRp[T]GpYTLGTPTYMAPEQAK | p[T163, T165, Y167, T168] | ||
| VMDFGLAp[YLLQES]R | p[Y154, S159] | ||
| TTC1888/apgM | Cofactor-independent phosphoglycerate mutase | LAEESALGLLTPVYPGLAPGpSGPGHLALFGYDPFR | pS70 |
| LTPAAIASYPMpYK | pY256 | ||
| TTC1904 | UDP-N-acetylmuramoylalanyl-d-glutamate-2,6-diaminopimelate | LGEEARPPVGHFpTTPEAPEVYAFLR | pT155, pT156 |
| TTC1957/bmrU | Protein bmrU | VLGVVPIGpSGNDFAR | pS86 |
| TTC1960 | Aspartate aminotransferase | AVRPp[SGAFYVLMDTS]PIAPDEVR | p[S318, Y322, T327, S328] |
| AVRPp[S]GAFpYVLMDTSPIAPDEVR | pY322, p[S318] | ||
| TT P0022 | Histidinol-phosphate aminotransferase | RALAEAHGVSPEQVAVGpTGp[T]SELIHR | pT78, p[T80] |
| TT P0024/phoA | Alkaline phosphatase | YPNGLINTYSLTSYVTEp[SS]AAGNAFSCGVK | p[S95, pS96] |
Sites of phosphorylation with 99% peptide identification confidence assigned by MaxQuant software are indicated in bold: pS, phosphoserine; pT, phosphothreonine; pY, phosphotyrosine. Ambiguous sites are indicated in bold with brackets as p[S], p[T], and p[Y]. Detailed information on the detected phosphopeptides can be found in supplemental Table S1.