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. 2013 Jul 3;12(10):2804–2819. doi: 10.1074/mcp.M112.025882

Fig. 6.

Fig. 6.

The effect of knockdown of BAG3 on proteasome activity and MG-132 sensitivity in HeLa cells. A, knockdown of BAG3 in HeLa cells was confirmed by Western blotting. B, quantitative analysis of BAG3 knockdown on apoptosis in HeLa cells. Data are expressed as the mean ± S.D. of the fractions of apoptotic cells from at least three experiments. C, knockdown of BAG3 resulted in a marked decrease in proteasome activity. D, knockdown of BAG3 increased the ubiquitin-tagged proteins in HeLa cells. Western blotting was carried out with anti-ubiquitin antibody using total cell lysates extracted from HeLa cells. Actin served as a loading control. Effect of knockdown of BAG3 on the chymotryptic, tryptic, and caspase-like activities of the (E) 26S or (F) 20S proteasome in HeLa cells, as measured using fluorogenic substrates. Experiments were repeated a minimum of three times. *p < 0.05 when compared with the control group. 1: untreated HeLa cells; 2: HeLa cells treated with control siRNA; 3: HeLa cells treated with BAG3 siRNA; 4: HeLa cells treated with MG-132; 5: HeLa cells treated with control siRNA and MG-132; 6: HeLa cells treated with BAG3 siRNA and MG-132.