Skip to main content
. 2013 Jul 3;12(10):2804–2819. doi: 10.1074/mcp.M112.025882

Fig. 7.

Fig. 7.

The effect of overexpression of BAG3 on proteasome activity and MG-132 sensitivity in HeLa cells. A, overexpression of BAG3 in HeLa cells was confirmed by Western blotting. B, overexpression of BAG3 reduces MG-132 sensitivity in HeLa cells. C, overexpression of BAG3 resulted in a marked increase in proteasome activity in MG-132-treated cells. D, overexpression of BAG3 decreased the ubiquitin-tagged proteins in MG-132-treated HeLa cells. Effect of overexpression of BAG3 on the chymotryptic, tryptic, and caspase-like activities of the (E) 26S or (F) 20S proteasome in HeLa cells, as measured using fluorogenic substrates. Experiments were repeated a minimum of three times. *p < 0.05 when compared with the control group. 1: untreated HeLa cells; 2: HeLa cells treated with blank vector; 3: HeLa cells treated with BAG3 plasmid; 4: HeLa cells treated with MG-132; 5: HeLa cells treated with blank vector and MG-132; 6: HeLa cells treated with BAG3 plasmid and MG-132.