Fig. 2.

Proteolysis of SLP76 molecules in BMMC. (A, B) BMMC (A) and thymocytes (B) were solubilized in LM lysis buffer or in SDS lysis buffer. Equal amounts of cell lysates were electrophoresed and Western blotted with the indicated antibodies. C, IgE anti-DNP-sensitized BMMC were challenged with DNP-HSA for the indicated times and solubilized in LM lysis buffer. Equal amounts of BMMC lysates were electrophoresed and Western blotted with anti-SLP76 and anti-actin antibodies. D, Equal amounts of proteins from a thymocyte LM lysate, a BMMC LM lysate or a mixture of both were incubated for 10 min on ice, electrophoresed and Western blotted with anti-SLP76 antibodies. E, BMMC were lysed in SDS lysis buffer or in LM lysis buffer containing no inhibitors (1), Roche mixture inhibitors 1× (2), Roche mixture inhibitors 10× (3), Sigma-Adlrich mixture 1× (4) or custom-made mixture inhibitors (21) (5). Lysates were electrophoresed and Western blotted with anti-SLP76 and anti-actin antibodies.