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Effect of the ECM in osteoprogenitor cell mineralization. A, Vehicle and activin A treated cultures were devitalized just before the onset of mineralization. The remaining ECMs were cultured with osteogenic medium (+ medium) and undifferentiated hMSC in osteogenic medium (+ hMSC). In parallel, hMSCs were also seeded directly on standard plastic cultures plates. B, Calcium content was assayed at the end of culture (n = 3). Value means ± S.D. ** p < 0.01; *** p < 0.001.
