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. 2013 Jun 13;12(10):2935–2951. doi: 10.1074/mcp.M113.030643

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 4. — Heat map of the consensus data. Agglomerative hierarchical clustering results were processed based on the differences of relative intensity values for glycan compositions between PSA and PSA high isoform. Orange color indicates a null difference of relative intensity between PSA and PSA high isoform, yellow indicates a positive difference, red indicates a negative difference. The N-glycan compositions of cluster 7 (80% of the total compounds) are similar between PSA and PSA high isoform. Compositions are more intense in PSA high isoform than PSA for the cluster 1 (Hex₅HexNAc₄dHexNeuAc₂, Hex₅HexNAc₄dHexNeuAc), cluster 3 (Hex₄HexNAc₅dHexNeuAc₂), cluster 4 (NeuAc₁Hex₅HexNAc₄NeuAc), cluster 6 (Hex₅HexNAc₄dHex, NeuAc₂Hex₅HexNAc₄NeuAc₂). Compositions are less intense in PSA high isoform than PSA for cluster 2 (Hex₄HexNAc₄dHexNeuAc, Hex₄HexNAc₃dHexNeuAc, Hex₆HexNAc₃NeuAc), cluster 5 (Hex₄HexNAc₄NeuAc, Hex₅HexNAc₂), cluster 8 (Hex₃Hex₅dHexNeuAc, Hex₄Hex₃NeuAc). In order to confirm the significance of the differences in N-glycan composition abundances between PSA and PSA high isoform, a W-test was used and revealed 8 significant compositions (indicated in bold).