Table II. Percentage of participating laboratories detecting N-glycan. The percentage of N-glycan detected has been calculated for the methods used (bottom-up, top-down, and PNGase). For example, the compound Hex₅HexNAc₄NeuAc₁ was detected by 82% of the total participating labs (18 out of 22 participating labs), by 71% of the participants using a bottom-up approach (12 out of 17 labs), and by 100% of the participants using top-down and PNGase approaches (4 out of 4 labs). The up-arrows indicate that the percentage of N-glycan detected per methodology is higher than the percentage of N-glycan detected for all the participants. The right-facing arrows indicate that both percentages are the same. The down-arrows indicate that the percentage of N-glycan detected per methodology is lower than the percentage of N-glycans detected by all the participating laboratories. The top-down and PNGase methodologies presented better detection efficiencies than the bottom-up approach for the major and intermediate N-glycans. The minor compounds did not present any such trend.

Class	Composition	Percentage of participating laboratories (%)	Bottom-up (%)	Top-down (%)	PNGAse (%)
Major glycan	Hex5HexNAc4dHex1NeuAc1	95	↓ 93	↑ 100	↑ 100
	Hex5HexNAc4dHex1NeuAc2	93	↓ 89	↑ 100	↑ 100
	Hex5HexNAc4NeuAc2	84	↓ 75	↑ 100	↑ 100
	Hex5HexNAc4NeuAc1	82	↓ 71	↑ 100	↑ 100
	Hex4HexNAc3dHex1NeuAc1	80	↓ 75	↑ 88	↑ 88
	Hex4HexNAc4dHex1NeuAc1	80	↓ 71	↑ 88	↑ 100
	Hex6HexNAc3NeuAc1	68	↓ 64	↑ 75	↑ 75
Intermediate N-glycan	Hex4HexNAc5dHex1NeuAc2	61	→ 61	↑ 63	↑ 63
	Hex5HexNAc4dHex1	61	↑ 64	↑ 75	↓ 38
	Hex4HexNAc5 NeuAc1dHex1	57	↓ 46	↑ 100	↓ 50
	Hex4HexNAc3NeuAc1	57	↓ 43	↑ 63	↑ 100
	Hex4HexNAc5NeuAc1	55	↓ 43	↑ 63	↑ 88
	Hex4HexNAc4NeuAc1	52	↓ 43	↑ 63	↑ 75
	Hex4HexNAc3dHex1	52	↓ 50	↓ 50	↑ 63
	Hex3HexNAc5dHex1NeuAc1	41	↓ 39	↑ 50	↓ 38
	Hex5HexNAc2	34	↓ 25	↓ 25	↑ 75
	Hex4HexNAc4dHex1	34	↑ 36	↓ 25	↑ 38
	Hex4HexNAc5NeuAc2	34	↓ 25	↑ 50	↑ 50
Minor N-glycan	Hex5HexNAc3NeuAc1	27	↓ 14	↑ 63	↑ 38
	Hex3HexNAc5NeuAc1	25	→ 25	→ 25	→ 25
	HexNAc5dHex1NeuAc1Hex4SO3	23	↓ 21	↓ 13	↑ 38
	Hex4HexNAc5dHex1	23	↑ 25	↑ 25	↓ 13
	Hex6HexNAc2	23	↓ 4	↑ 25	↑ 88
	Hex6HexNAc3	23	↓ 21	↑ 50	↓ 0
	Hex5HexNAc4	20	↑ 25	↓ 0	↑ 25
	Hex5HexNAc5NeuAc2	20	↓ 11	↑ 63	↓ 13
	Hex4HexNAc5	20	↓ 11	↑ 50	↑ 25
	Hex5HexNAc5dHex1NeuAc1	20	↓ 18	↑ 50	↓ 0
	Hex3HexNAc4dHex1NeuAc1	20	↓ 14	↑ 38	↑ 25
	Hex6HexNAc3dHex1NeuAc1	20	↑ 29	↓ 0	↓ 13
	NeuAc1Hex6HexNAc4	18	↓ 14	↑ 50	↓ 0
	Hex4HexNAc2	18	↓ 14	↓ 0	↑ 50
	Hex4HexNAc5dHex1SO3	18	↑ 21	↓ 0	↑ 25
	Hex5HexNAc4dHex2NeuAc1	18	↑ 21	↓ 0	↑ 25
	Hex3HexNAc6dHex1NeuAc1	16	↓ 11	↑ 38	↓ 13
	Hex5HexNAc5	14	↑ 14	↓ 0	↑ 25
	Hex6HexNAc5dHex1NeuAc2	14	↓ 11	↓ 13	↑ 25
	Hex6HexNAc4NeuAc2	14	↓ 4	↑ 50	↓ 13
	Hex6HexNAc5dHex1NeuAc3	14	↓ 7	↑ 25	↑ 25
	Hex5HexNAc5NeuAc1	14	↑ 14	↑ 25	↓ 0
	Hex4HexNAc5NeuAc1SO3	14	↑ 14	↓ 0	↑ 25
	Hex3HexNAc6NeuAc1	14	↑ 14	↓ 13	↓ 13
	Hex3HexNAc3dHex1	11	↑ 18	↓ 0	↓ 0
	Hex3HexNAc4dHex1	11	↑ 18	↓ 0	↓ 0
	Hex5HexNAc5 dHex1	11	↓ 4	↑ 50	↓ 0
	Hex6HexNAc3 dHex1	11	↓ 4	↑ 38	↑ 13
	Hex3HexNAc3dHex1NeuAc1	9	↑ 14	↓ 0	↓ 0
	Hex3HexNAc4dHex1SO3	9	↓ 7	↑ 25	↓ 0
	Hex3HexNAc5SO3	9	↓ 7	↓ 0	↑ 25
	Hex5HexNAc5NeuAc1dHex2	9	↑ 11	↓ 0	↑ 13
	Hex3HexNAc5(SO3)2	7	↓ 4	↓ 0	↑ 25
	Hex4HexNAc4NeuAc1SO3	7	↓ 4	↓ 0	↑ 25
	Hex6HexNAc4dHex1	7	↓ 0	↑ 38	↓ 0
	Hex6HexNAc5	7	↓ 0	↑ 13	↑ 25
	HexNAc1	7	↓ 0	↑ 38	↓0
	Hex7HexNAc3NeuAc1	7	↑ 11	↓ 0	↓ 0
	Hex4HexNAc5dHex2NeuAc1	7	↑ 11	↓ 0	↓ 0
	Hex4HexNAc4NeuAc2	5	↓ 0	↓ 0	↑ 25
	Hex5HexNAc4dHex1NeuAc1NeuGc1	5	↓ 0	↑ 25	↓ 0
	Hex5HexNAc4SO3	5	↓ 4	↓ 0	↑ 13
	Hex4HexNAc4NeuAc2	5	↓ 0	↓ 0	↑ 25