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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Angiogenesis. 2013 Aug 17;16(4):861–875. doi: 10.1007/s10456-013-9361-x

Fig. 1. Specific overexpression of Spry4 in endothelial cells inhibits retina angiogenesis.

Fig. 1

Representative photographs show IB4 staining of different parts of retinas from P5 wild-type (WT) and Spry4;VE-cad-Cre transgenic mice. Spry4 has no effect on primary branching from the center of retina (A), but significantly represses secondary branching (B) and vessel density (C). (n = 6; * p < 0.05). Murine Primary endothelial cells were isolated from the lungs of Spry4;VE-cad-Cre (Spry4TG), Spry4f/f:VE-cad-Cre (Spry4−/−) mice and their Cre-negative littermates. Deletion or overexpression of Spry4 was achieved by transducing cells in vitro with AdCre. Cells were use 24–48 h after Cre-mediated recombination. Representative immunoblots are shown. Equal amount of cell lysate were loaded for all samples. Spry4 overexpression inhibits phosphorylation of c-Src, AKT and ERK in murine primary endothelial cells (D), whereas Spry4 knockout enhances their phosphorylation (E).