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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Angiogenesis. 2013 Aug 17;16(4):861–875. doi: 10.1007/s10456-013-9361-x

Fig. 4. Spry4 modulates integrin β3 expression and its interaction with VEGFR-2 via c-Src.

Fig. 4

(A) HUVECs grown on VTN were transduced with AdLacZ or AdSpry4 with or without AdCASrc, and lysed 24 h later and subjected to immunoblotting with the indicated antibodies. (B) HUVECs grown on VTN were transduced with LentiNT or LentishSpry4, and selected with puromycin (0.5 μg/ml) for 5 days. After transduction with AdLacZ or AdDNSrc, the cells were lysed 24 h later and subjected to immunoblotting with the indicated antibodies. (C, D) Quantification of relative integrin β3 expression normalized to tubulin and relative to LacZ or NT controls; n = 6; data are means ± S.D. ** p < 0.01. (E, F) Cell lysates were immunoprecipitated with anti-VEGFR-2 or anti-integrin β3 antibodies. Immune complexes were then resolved by SDS-PAGE and immunoblotted with VEGFR-2, integrin β3 and Src specific antibodies. Representative immunoblots are shown. Equal amount of immune complexes and cell lysates were loaded for all samples.