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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Angiogenesis. 2013 Aug 17;16(4):861–875. doi: 10.1007/s10456-013-9361-x

Fig. 5. Spry4 regulates the protein stability of integrin β3 via c-Src.

Fig. 5

(A) Real-time qPCR analyses reveals no effect of Spry4 on integrin β3 mRNA levels; n = 3; data are means ± S.D. (B) RT-PCR analysis show that mRNA levels of integrin β3 in HUVECs that either overexpressing or depleted of Spry4 by shRNAs are unaffected. (C) HUVECs were transduced as indicated, and cell lysates were prepared at different time points as indicated after cycloheximide (10 μg/ml) treatment. Equal amounts of cell lysate were loaded and analyzed by immunoblotting. Representative immunoblots are shown. (D, E) Quantification of relative integrin β3 protein levels normalized to tubulin and relative to the levels at 0 hr of each group; n = 3; data are means ± S.D. (F) Subcellular factions of HUVECs either overexpressing or depleted of Spry4 by shRNAs were prepared and analyzed by immunoblotting. Representative immunoblots are shown. Equal amount of cell lysate were loaded for all samples.