Fig. 3.

Neutrophils that infiltrated the brain and blood vessel endothelial cells expressed MMP9 at 24 hour after TBI. hMSCs (10⁶ cells/mouse) or TSG-6 protein (50 μg/mouse) were administrated 6 hour after TBI. (A) Representative sections showing double-immunofluorescence labeling of cortical sections from the injured mouse treated with vehicle, hMSC or TSG-6 at 24 hour after TBI. The images were taken from superficial pericontusional area of cortex. Co-labeling of MMP9 (red) with marker for neutrophils (Ly6G/Ly6C, green). Left: ×20 magnification (scale bars=100 μm), Right: ×60 magnification (scale bars = 50 μm). (B) Images of representative sections showing double-immunofluorescence detection of cortex sections from the injured mice that received vehicle, hMSCs or TSG-6 at 24 hour after TBI. The images were taken from superficial pericontusional area of cortex. Co-labeling of MMP9 (red) with von Willibrand Factor in blood vessel endothelial cells (vWF, green). Left: ×20 magnification (scale bars=100 μm), Right: ×60 magnification (scale bars = 50 μm).