Table 1. Antibodies used for immunostaining.
| Antibody | Antigen | Host | Clone | Company | Dilution | Target |
|---|---|---|---|---|---|---|
| NeuN | purified cell nuclei from mouse brain |
mouse | A60 | Millipore | 1000 | neurons |
| Ly6G/Ly6C | mouse Ly-6G and Ly-6C |
rat | RB6-8C5 | BD Biosciences |
100 | neutrophils |
| MMP9 | mouse MMP9 | goat | polyclonal (AF909) |
R&D systems | 500 | MMP9 |
| vWF | human von Willebrand Factor |
rabbit | Polyclonal (AB7356) |
Millipore | 50 | brain blood vessels endothelial cells |
| DCX | Human Doublecortin |
goat | C18 (sc-8066) | Santa Cruz | 250 | New born neurons |
| mouse IgG | mouse IgG | goat | Invitrogen | 500 | Alexa 488 labeled 2nd antibody | |
| rat IgG | rat IgG | goat | Invitrogen | 500 | Alexa 488 labeled 2nd antibody | |
| goat IgG | goat IgG | donkey | Invitrogen | 500 | Alexa 592 labeled 2nd antibody | |
| rabbit IgG | rabbit IgG | goat | Invitrogen | 500 | Alexa 488 labeled 2nd antibody | |
| Goat IgG | Goat IgG | horse | Vector Labs | 200 | Biotin labeled 2nd antibody |