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Optical absorption spectra of the WT and mutant P450cam enzymes in 50 mM potassium phosphate buffer , pH 7.4 with 3 mM D-camphor in the absence (A) or presence (B) of 400 mM KCl. WT P450cam is shown in orange, mutants L358A (green), K178G (black) L358A/K178G (magenta) and L358A/K178G/D178N (cyan) are overlaid on the WT spectra.
