Figure 2.

Female responses to direct and phenotypic familiarity of males. (a) Proportion of courtship rejected by females. From left to right: ‘direct familiarity’ experiment in which wild-type females were presented with a previous mate and novel mate (n = 43); ‘phenotypic novelty’ experiment in which wild-type females were presented with a full-sibling of their first mate who had developed in the same vial as that male (phenotypically familiar), and one male who was unrelated to the first mate and from a different vial (phenotypically novel; n = 59); ‘phenotypic novelty’ experiment in which Orco¹ females were presented with a brother of their first mate who had developed in the same vial as that male (phenotypically familiar), and one male who was unrelated to their first mate and from a different vial (phenotypically novel; n = 56). Dashed lines separate different experiments. Asterisk (*) equals 0.05 < p < 0.10 for tests for difference in proportion of courtship rejected between familiar and novel males. p-values from left to right are 0.088, 0.082 and 0.176. (b) Cumulative survival functions for wild-type females. Likelihood ratio tests revealed a significant difference in remating latency with phenotypically familiar and phenotypically novel mates (n = 40, , p = 0.019). (c) Proportion of females mating with a novel male first in the phenotypic novelty experiment. Focal females were either wild-type or Orco¹ and were presented with a brother belonging to the same local environment as the first mate (phenotypically familiar) and one non-sibling belonging to a different local environment as the first mate. (d) Cumulative survival functions for Orco¹ females. Likelihood ratio tests revealed no significant difference in remating latency with phenotypically familiar and novel mates (n = 35, , p = 0.344). Error bars denote s.e.