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STORM localization of collagen α3α4α5 (IV)-NC1 (A) and collagen α3α4α5 (IV)-periN (B) domains along with agrinC show the position of the collagen α3α4α5 (IV) network towards the center of the GBM. In contrast, collagen α1α1α2 (IV) labeling (C) is restricted to the endothelial side of the GBM. (D) Nidogen localizes in the center of the GBM. Scale bar: 200 nm.
