Figure 6.

Rictor regulates induction of prosurvival genes and suppression of proapoptotic genes. (A) Relative expression of Bcl2 family genes ex vivo. Mature (AA4.1⁻) B cells were purified from Vav-Cre Rictor^fl/fl (cKO) or control mice and were analyzed by qRT²-PCR. Shown are the mean (± SEM) concentrations of mRNA in cKO relative to WT, averaging 5 mice. (B) qRT²-PCR was performed on RNA from purified B cells of Cre-ER^T2 Rictor^fl/fl or control mice, pretreated with tamoxifen (2 weeks; before observed distortion in B-cell populations) as in Figure 3A, after culture (24 hours) with anti-IgM or BAFF. Shown are the means (±SEM) of relative expression for the indicated mRNA averaging results from 3 (BAFF) or 4 (anti-IgM) independent experiments.