Table IV.
Summary of minibioreactor properties reported in peer reviewed publications.
| Refs. | kLa (h-1) | Volume (mL) | Filling height (mm) | Mixing | Cell type | Measured | Controlled | Notes |
|---|---|---|---|---|---|---|---|---|
| Kostov et al. (2001) | 9.8–21 | 2 | 20 | Magnetic stir bar + sparging | E. coli | DO, OD, pH | T | Foaming problems |
| Lamping et al. (2003) | 70–360, 100 used | 6 | 30 | Impeller + sparging | E. coli | DO, OD, pH | T | Difficult to multiplex |
| Puskeiler et al. (2005) | 180–1,440 | 5–12 | 32 (at 10 mL) | Impeller + sparging | E. coli | T, pH | No real-time OD, DO, automated sampling for OD | |
| Weuster-Botz et al. (2005) | 12 | 38 | Impeller + sparging | E. coli | DO, OD, pH | T | Multiplexed DO monitoring added, automated sampling for OD | |
| Harms et al. (2006) | 68–300 | 1 | 5.2 | Impeller + sparging | E. coli | DO, OD, pH, GFP | Problems measuring OD, GFP, kLa inconsistency at higher speeds | |
| Tang et al. (2006) | 6 | 31.4 | Agitated | Shewanella oneidensis | DO, pH | T, DO, pH | Manual sampling for OD, foaming problems | |
| Isett et al. (2007) | 32.6–56.1 | 5 | 26.2 | Agitated | S. cerevisiae, E. coli, P. pastoris | DO, pH | T, DO, pH | Manual sampling for OD, foaming problems |
| Chen et al. (2009) | 6 | 31.4 | Agitated | CHO | DO, pH | T | Manual sampling for OD, foaming problems | |
| Hortsch et al. (2010) | 180 | 10 | 3.2 | Impeller | Streptomyces tendae | DO, pH | T | Manual sampling for OD, product concentration |
| Klein et al. (2012) | 26.8–48.5 | 10 | 50 | Magnetic stir disc | Schizosaccharomyces pombe | DO | T | Chemostat, off gas and liquid effluent analysis |