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. Author manuscript; available in PMC: 2014 Aug 26.
Published in final edited form as: Dev Cell. 2013 Aug 15;26(4):358–368. doi: 10.1016/j.devcel.2013.07.014

Fig. 2. Phospho(T94)Tctex-1 is a downstream effector of IGF-1 induced ciliary resorption.

Fig. 2

(A, B) Representative images of cells immunolabeled for phospho(T94)Tctex-1, Ac-Tub, and γ-Tub in quiescent RPE-1 cells (A; 0 hr) or IGF-1 treated cells (B; 2 hr). (C, D) Representative images of cilia in IGF-1 stimulated RPE-1 cells incubated with control HA Ab (C) or anti-IGF-1R αIR3 Ab (D), and immunolabeled with indicated Abs. (E) Percentages of control- and Tctex-1-sh/GFP- transfected 3T3 cells displaying cilia after serum or IGF-1 treatments. (F) Biphasic ciliary resorption profiles of IGF-1 treated cells that were non-transfected, or transfected with IGF-1R-sh/GFP alone (−) or together with Tctex-1 mutant T94E or T94A. Data are presented as mean ± SEM (**p<0.01; *p<0.05; one-way ANOVA; n=3 experiments). Scale bars: 2 µm (A, B).