Figure 5. ADSC inhibit expression of costimulatory molecules by DC in vitro.
(A) In an MLR culture, naïve CD4+ T cells from the spleens of C57BL/6 mice were co-cultured with mature dendritic cells from CBA/J mice in the presence of 1×105 ADSC with contact or ADSC separated by a permeable membrane (transwell) for 4 days. Cells were analyzed by flow cytometry. (A) Histogram showing the expression of CD40, Cd80 and CD86 [Isotype control-tinted gray; CD4+DC+ADSC black line and CD4+DC+ADSC (Transwell)]. Data were analyzed using FlowJo.