Figure 1. Increases in [Ca²⁺]_i in response to NaCl and H₂O₂ treatments.

(A and C) Increases in [Ca²⁺]_i induced by several concentrations of NaCl (A) and H₂O₂ (C) in Arabidopsis. Seedlings expressing aequorin and grown for 7 days were treated with solutions containing several concentrations of NaCl or H₂O₂, and aequorin images were taken every 10 sec for 500 sec. Data for four independent experiments are shown (mean ± sem; n = 64).

(B and D) Time courses of increases in [Ca²⁺]_i induced by 200 mM NaCl (B) or 4 mM H₂O₂ (D). Seedlings grown for 7 days were treated with NaCl and H₂O₂ at time zero, and aequorin images were taken every 10 sec. Representative recordings from individual seedlings were shown. Similar results were seen in six independent experiments using 128 seedlings.