Cells were treated with CO-OCS or SO-OCS (40 µM) for 72-h. Thereafter, cells were collected by trypsinization, stained with propidium iodide and the DNA content was measured by flow cytometry. The percentage of cells within G0/G1, S, G2/M, were determined. CO-OCS accumulated cells in the G0/G1 and G2/M phases in MCF-7cells (A), and in G2/M phase in MDA-MB-231 cells (B). However, SO-OCS accumulated cells in G2/M phase in both MCF-7 and MDA-MB-231 cells (D, E). No synchronization was observed in MCF-10A cells (C, F). The results represent means ± SD of three independent experiments. Inserts: traces representing distribution of the cells in the cell cycle. Asterisks denote statistical significance as compared to control cells *
p<0.05, **
p<0.01, ***
p<0.001.