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. 2013 Oct 4;8(10):e76129. doi: 10.1371/journal.pone.0076129

Figure 6. Heat acclimation induces transient reduction in membrane β-catenin and synaptophysin.

Figure 6

Membrane cellular fraction extracts were separated on SDS-PAGE gels and analyzed using western blotting. After the injury a significant reduction in membrane bound β-catenin were seen at 72 h post injury in heat acclimated (HA) mice and after 7 days in normothermic (NT) mice. Membrane bound β-catenin levels were recovered by 7 days after injury (A) Blots of membrane extracts show significant reduction in synaptophysin levels in both groups at 72 h after injury with a recovery seen only in HA mice by 7 days post injury (B). The absence of synaptophysin in the cytosol verifies membrane protein enrichment. Values represent the mean ± SEM. n = 5–6 per group. *p<0.05 vs. NT mice, **p<0.05 vs. sham mice,within the same group.