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. 2013 Oct 4;8(10):e77182. doi: 10.1371/journal.pone.0077182

Figure 1. Cell age-dependent effects of glutamate, hypoxia on the expression of EPO, glutamine synthetase (GS), EPO release and EPO mRNA expression in rat APC.

Figure 1

(A) Western blot analysis of GS and EPO in cell homogenates prepared from 7- (DIV7) and 21-day-old (DIV21) rat APC under normal and hypoxic conditions with or without exposure to Glu and EPO. (B) The cell-age-dependent release of EPO into the culture medium under normoxia (N, white bars) and 24h or 48h hypoxia (H, grey bars) in 7-,15- and 21-day old APC. (C) The effect of EPO treatment (5U/ml) on the expression of EPO mRNA in early (7-day-old) APC under normoxia (N, white bars) and hypoxia (H, grey bars). (D) The effect of EPO on the expression of EPO mRNA in prolonged (21-day-old) APC under normoxic (N) and hypoxic (H) culture conditions. Data are normalized to normoxic control (N) *, p<0.05, **, p<0.01, ***, p<0.001.