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. 2013 Oct 4;8(10):e77182. doi: 10.1371/journal.pone.0077182

Figure 3. Cell age-dependent Glu-uptake and the effects of EPO on the expression of GLAST by astroglial primary cultures (APC) under normoxic and hypoxic culture conditions.

Figure 3

(A) Quantification of GLAST-positive astrocytes (GLAST+/GFAP+cells) under normoxia shows a significant (**p<0.01; ***p<0.001) increase in GLAST+/GFAP+cells upon EPO-treatment in both young and aged APC (D7 and D21); (B) hypoxia enhanced the effect of EPO on GLAST/GFAP+ cells. In both young and aged cells the number of GLAST/GFAP cells was increased (**p<0.01 at d7 and ***p<0.001 at d21). (C) The uptake of 1 mM glutamate by APC (shown in absolute values) was increased on day 14 in comparison with day 7 under normoxia (white bars, **p<0.01) and hypoxia (grey bars, ***p<0.001) and strongly decreased in 21-day old cultures (d21) exposed to hypoxia (grey bars) when compared to those from day 7 in hypoxia (grey bars d21vs. d7, p***<0.001).