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. 2013 Oct 4;8(10):e77182. doi: 10.1371/journal.pone.0077182

Figure 6. Cell age- and concentration-dependent effects of EPO on glutamine synthetase activity.

Figure 6

(A) DIV7 APC under normoxia treated with (+1mM Glu) or without (-Glu) and 1 or 5U/ml EPO (1UE or 5 UE respectively); (B) DIV7 APC under 24h normoxia /white bars) or hypoxia (grey bars) treated with (+Glu) or without (-Glu) and 1 or 5U/ml EPO (1UE or 5 UE respectively); (C) DIV14 APC under normoxia treated with (+Glu) or without (-Glu) and 1 or 5U/ml EPO; (D) DIV14 APC upon 24h normoxia /white bars) or hypoxia (grey bars) treated with (+Glu) or without (-Glu) and 1 or 5U/ml EPO (1UE or 5 UE respectively); (E) DIV21 APC under normoxia treated with (+Glu) or without (-Glu) and 1 or 5U/ml EPO (1UE or 5 UE respectively); (F) DIV21 APC under 24h normoxia /white bars) or hypoxia (grey bars) treated with (+Glu) or without (-Glu) and 1 or 5U/ml EPO (1UE or 5 UE respectively). At all three time points in culture, Glu increased the activity of GS when compared to respective controls culture without Glu (-Glu). Treatment with EPO increased the glutamate-induced activation of GS in concentration-dependent manner when compared to control culture (cf. +Glu vs +Glu+1U E and +Glu+5U E). *, p<0.05, **, p<0.01, ***, p<0.001.