Figure 4. Time course of inflammatory cell accumulation in rat tibialis anterior muscle following bupivacaine injection.

Transverse sections (10 µm) were immunoassayed with specific antibodies against neutrophils (W3/13) (A), ED1⁺-macrophages (B), or ED2⁺-macrophages (C). Labeled cells were counted at 400X magnification, and expressed as a number of cells/mm³. Values are mean ± SEM (n = 7-8 rats per dietary group/time point). As a control, values for neutrophils and macrophages at T0 are indicated by the dotted line. Groups bearing different letters for a given time point are significantly different (p≤0.05). C, casein; CP, cod protein; C⁺, casein supplemented with L-arginine, glycine, L-taurine and L-lysine.