Table 4. Secondary structures of WT-hAS3MT and the mutants estimated from ATR-FTIR spectroscopy.
α-helix% | β-pleated% | β-turn% | Random% | |
Y59A | 38.9±3.1 | 17.0±0.8 | 14.2±0.8 | 29.9±2.0 |
G78A | 26.4±0.5 | 33.8±0.3 | 13.4±0.3 | 26.3±0.8 |
S79A | 29.9±1.0 | 29.9±0.4 | 13.1±0.3 | 27.1±0.3 |
M103A | 26.0±1.7 | 32.7±2.5 | 16.3±1.7 | 24.9±1.1 |
Q107A | 26.7±0.8 | 30.6±1.3 | 15.3±0.3 | 27.4±0.5 |
I136A | 24.1±1.8 | 43.6±1.7 | 10.8±0.9 | 21.5±0.6 |
E137A | 27.3±2.3 | 27.8±2.1 | 15.5±1.1 | 29.4±1.6 |
WT | 26.6±3.6 | 20.7±4.6 | 24.2±3.2 | 28.5±4.9 |