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. 2013 Oct 4;8(10):e77012. doi: 10.1371/journal.pone.0077012

Figure 5. Decreased stratifin expression results in increased exchange of plakophilin-3/GFP at the plasma membrane.

Figure 5

A. Cell lysate prepared from A431 cells expressing a control shRNA and A431 cells stably expressing shSFN were immunoblotted with anti stratifin and anti beta-tubulin. B. FRAP analysis of A431 cells expressing plakophilin-3/GFP together with a control shRNA or shRNA targeting stratifin (left panel) and A431 cells expressing plakophilin-3 ΔSL/GFP together with control shRNA or shRNA targeting stratifin (right panel). For each graph, the relative fluorescence intensity is plotted and the error bars represent 95% confidence interval. C. Representative images (Movies S1 and S2) from the FRAP experiment depicted in the left panel of B.