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. 2013 Oct 4;8(10):e75182. doi: 10.1371/journal.pone.0075182

Figure 6. Ansamitocin P3 treatment activated p53.

Figure 6

(A) Ansamitocin P3 treatment caused an increase in the expression level of p53 in MCF-7 cells. Lanes 1, 2, 3, and 4 represent control, 100 and 150 pM of ansamitocin P3 and 25 nM vinblastine treated cells, respectively. β-actin was used as loading control. Ansamitocin P3 treatment increased the nuclear accumulation of p53 (B) and p21 (C). MCF-7 cells were incubated without or with different concentrations (20, 50 and 100 pM) of ansamitocin P3 for 24 h. Scale bar is 10 µm.