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. 2013 Oct 4;8(10):e75182. doi: 10.1371/journal.pone.0075182

Figure 7. Ansamitocin P3 quenched tryptophan fluorescence of tubulin.

Figure 7

(A) Effects of ansamitocin P3 on the intrinsic tryptophan fluorescence of tubulin are shown. Tubulin was incubated in 25 mM PIPES buffer (pH 6.8) without (▪) and with different concentrations 0.5 (○), 1 (▴), 2 (∇), 3 (♦), 5 (×), 7 (Δ), 10 (□) and 15 (•) µM of ansamitocin P3, respectively. (B) The dissociation constant was determined from the fluorescence change data using Graph Pad Prism 5 software The Kd was average of 5 data sets.