Figure 8. Ansamitocin P3 suppressed chemical modification of cysteine residues of tubulin by DTNB.

(A) Kinetics of modification of tubulin by DTNB in the absence (▪) and presence of 3 µM (▴), 5 µM (•) of ansamitocin P3 are shown. The absorbance was monitored at 412 nm. (B) The pseudo first order plots of sulfhydryl modifications in the absence (▪) and presence of 3 µM (▴) and 5 µM (•) ansamitocin P3 are shown. One of three experiments is shown.