Table 1. Parameters from Fitting of Titration Data.
Two-State Model | |||
Titrant | K d;app or (µM) | λf or λP(nm) | λb or λC•P(nm) |
Maltose ([C]T = 0)a | 1.2 ± 0.1 | 345.8 ± 0.1 | 350.0 ± 0.1 |
Maltose ([C]T = 100 g/L) | 1.9 ± 0.3 | 344.0 ± 0.1 | 349.8 ± 0.2 |
Maltose ([C]T = 200 g/L) | 3.9 ± 0.2 | 343.0 ± 0.1 | 349.9 ± 0.1 |
Maltose ([C]T = 300 g/L) | 8 ± 1 | 343.0 ± 0.1 | 350.3 ± 0.1 |
Ficoll ([L]T = 0)a | 2000 ± 500 | 345.6 ± 0.1 | 341.5 ± 0.4 |
Three-State Model | |||
K d (µM) (µM) | λP (nm) | λb (nm) λC•P (nm) | |
Maltose or Ficoll | 1.3 ± 0.1 | 345.8 ± 0.1 | 350.0 ± 0.1 |
1500 ± 200 | 341.8 ± 0.2 |
Bold entries from these two isolated titration experiments can be compared with the corresponding parameters in the global fit to the three-state model.