Figure 3. Identification and optimization of critical media composition for the production of lycopene in PTS01 strain.

(A) Half-normal probability plot of the results of minimal resolution IV experiment design (The details of the experiment design were shown in Table S1 in File S1). In the half-normal probability plot, the line was called the near zero line. The estimated effect of an unimportant factor will typically be on or close to a near-zero line, while the estimated effect of an important factor will typically be displaced well off the line. (B) RSM plot of lycopene production versus concentrations of glycerol and KH₂PO₄ of PTS01.