Figure 7. Media optimization of amorpha-1,4-diene production in PTS03 strain.

(A) Screening of key factors for amorpha-1,4-diene production (The factors share the same denotations as those described in Figure 3A. In the figure, A, B and E are dominant factors with positive effects on the production of amorpha-1.4-diene, therefore they were selected as key factors for further optimization). (B) Production of amorpha-1,4-diene versus glycerol concentration and inducer dosage. (C) Production of amorpha-1,4-diene versus KH₂PO₄ concentration and inducer dosage. (D) Comparison of amorpha-1,4-diene production in 2xPY and OPT2 media for the MG02 and PTS03 strains. The details of the experiment design are described in Supplementary Table S6 and S7 in File S1.