Figure 1. Reactive oxygen species (ROS) mediates IH-induced HIF-2α degradation.

A. Acontiase activity levels were monitored as an index of ROS generation in cells exposed to normoxia (N) or IH with and without MnTmPyP (50 µM), an anti-oxidant. B. Representative immunoblot of HIF-2α in PC12 cells exposed to normoxia (N) or to 60 cycles of intermittent hypoxia (IH) with and without MnTmPyP. C. Effect of H₂O₂ on HIF-2α expression in PC12 cells exposed to normoxia with and without PEG-catalase (300 U/ml). *p<0.05. n.s. not significant p>0.05.