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. 2013 Oct 4;8(10):e75838. doi: 10.1371/journal.pone.0075838

Figure 2. Role of NADPH oxidases in IH-induced HIF-2α degradation.

Figure 2

A. Effect of NADPH oxidase (Nox) inhibitors Apocynin (Apo, 1 mM) and AEBSF (15 µM) on HIF-2α protein following exposure to IH. B. HIF-2α expression in PC12 cells transfected with Nox2 and Nox4 siRNA and exposed to normoxia (N) or IH. Tubulin expression was monitored as control for protein loading. Bottom panels of A and B represent average data of densitometric analysis of the immunoblots presented as mean ± S.E.M from three independent experiments. *p<0.05; n.s. not significant, p>0.05.