Figure 3. Spectrum analysis of the cytochrome c content of WT and fur mutant strains under aerobic and anaerobic conditions.

The reduced-minus-oxidized difference spectra of equal amounts of total protein from the WT and fur mutant strains treated with sodium dithionite were recorded. The absorbance levels of the corresponding untreated strains were set as a control. Line 1 Absorbance of the aerobically grown WT strain. Line 2 Absorbance of the aerobically grown fur mutant strain. Line 3 Absorbance of the anaerobically grown WT strain. Line 4 Absorbance of the anaerobically grown fur mutant strain.