Figure 7. Peak Intensity of synthetic Hepcidin-25 of Peptides International (Vendor A; top panel) and Bachem (Vendor B; bottom panel) spiked to blank serum (to obtain a 10 nM concentration) and measured by WCX-TOF MS, using hepcidin-25⁺⁴⁰ from Peptides International as internal standard.

The theoretical concentrations of hepcidin-25 used in these experiments were adjusted towards 100% peptide content, based on the information provide in the package inserts of the respective Vendors (see Table 1). Hep-25, m/z 2789.4; Hep-25⁺⁴⁰ (internal standard), m/z 2829.4.