Table 1. Characteristics of commercial hepcidin peptides used.

Hepcidin peptide	Commercial source	Product no	Lot number	MW (Da)	pI	Peptide content	Purity
Heavy hepcidin-25	PI*	n.a.	221-006221	2829.4†	8.22	100%∧	>97.8%
Hepcidin-25	PI*B	PLP-4392-s	6103103005438	2789.4	8.22	100%∧68.6%	>98%>95%
Hepcidin-24	PI*	n.a.	740-609191	2673.9	8.51	100%∧	96.3%
Hepcidin-22#	PI	PLP-3776 PI	001055V	2436.1	8.53	74.5%	96.8%
Hepcidin-20#	PI	PLP-3777-PI	001041V	2191.8	8.53	67.6%	96.1%

^*catalog product of Peptide International but manufactured by Peptide Institute (Osaka, Japan);

^∧vialed at 100% peptide content;

^†due to an isotope content of 98% the actual mass is 1 Da less than the theoretical mass of this peptide.

^#delivered as 0.10 mg per vial; personal communication revealed that ≈ 0.11 mg was pipetted in each vial. PI, Peptide International (Louisville, KY, USA); B, Bachem LTD (St. Helens, UK); n.a., not applicable.