Table 2. Reproducibility of the measurement of hepcidin-25 and its isoforms of the improved hepcidin assay with the novel hepcidin-25⁺⁴⁰ as internal standard.^* .

	Intra-run (n = 8)		Inter-run (n = 8)
Hepcidin form	Level (nM)	CV(%)	Level (nM)	CV %
Hepcidin-25	2.6	2.7	2.6	8.3
	3.5	3.3	10.5	4.6
	7.4	3.5
	10.3	2.1
Hepcidin-24	1.4	8.2	2.3	16.8
	2.3	4.0
	2.4	2.9
Hepcidin-22	1.4	8.6	1.4	11.6
	1.7	7.8
Hepcidin-20	2.3	7.6	2.5	13.7
	4.9	4.2
	5.8	6.0

^*Heparin samples used for intra-run experiments are the high and low QC samples and samples from nephrology patients (n = 2). Heparin samples used for inter-run experiments are the high and low QC samples.