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(a) Brightfield images of a 3dpf zebrafish ventricle during systole with overlaid with velocity vectors calculated using PIV. Contours of (b) dv/dx, (c) du/dy and (d) the calculated wall shear rate (s−1) also overlaid with velocity vectors. Shear is concentrated in the region of the ventricular bulbar valve with the majority of the remainder of the heart experiencing comparatively low shear.
