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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Cancer Res. 2013 Aug 1;73(19):6068–6079. doi: 10.1158/0008-5472.CAN-13-0882

Figure 6. ERG and LEF1 induces EMT and are dys-regulated in prostate cancer.

Figure 6

A. ERG overexpression induces EMT of prostate epithelial cells. Phase-contrast microscopy images of stable control and ERG-expressing DU145 cells are shown. ERG-expressing cells lost cell-to-cell contact and showed a spindle-like shape.

B. The expression of epithelial marksis decreased by ERG and LEF1. QRT-PCR analysis of E-cadherin and Claudin-1 were carried out in control and DU145 cells with ERG and LEF1 overexpression.

C. ERG and LEF1 inhibit the expression of epithelial marks. Stable control, ERG-expressing, and LEF1-expressing DU145 cells were analyzed by western blot.

D. Categorization of ERG- and ERG+ prostate cancer specimens. A total of 472 primary prostate cancer tissues were separated into ERG- and ERG+ groups based on the expression level of ERG gene (52).

E. LEF1 is significantly up-regulated in the ERG+ prostate cancers. Boxplot analysis shows the expression level of ERG and LEF1 in the ERG- and ERG+ prostate cancer specimens.