Figure 5. Effect of delphinidin treatment on markers of proliferation and apoptosis in tumors of athymic nude mice implanted with NSCLC cells.

Athymic nude mice were implanted with [A] NCI-H441, and [B] SK-MES-1 cells. Mice were treated with delphinidin, tumor tissues were collected in 10% formalin and blocks were prepared in paraffin and immunostaining of Ki67, PCNA and cleaved caspase-3 were performed as described in “Materials and Methods”. Photomicrographs show representative pictures from three independent tumor samples. Bar = 20 µm.