Figure 6. Effect of delphinidin treatment on markers of angiogenesis in tumors of athymic nude mice implanted with NSCLC cells.

Athymic nude mice were implanted with [A] NCI-H441, and [B] SK-MES-1 cells. Mice were treated with delphinidin, tumor tissues were collected in 10% formalin and blocks were prepared in paraffin and immunofluorescence of CD31 and immunohistochemistry of VEGF were performed as described in “Materials and Methods”. Photomicrographs show representative pictures from three independent tumor samples. Bar = 20 µm.