Skip to main content
. 2013 Mar;1828(3):956–966. doi: 10.1016/j.bbamem.2012.11.030

Fig. 2.

Fig. 2

Isolation and analysis of DSS-cross-linked stomatin complexes. (A) Stripped ghosts were cross-linked with either 0.8 mM EGS (left side) or DSS (right side), and stomatin-complexes were isolated as before (see Fig. 1). Elution fractions were analysed by 10% SDS-PAGE (prolonged running time 24 h at 70 V), silver staining, and mass spectrometry of excised bands, as indicated ( > 500 kDa, 500 kDa, and 300 kDa). (B) Major components of DSS-cross-linked stomatin-complexes are band 3 and GLUT1. Semi-quantitative data are given in AvTIC units. M, marker; 1–5 and 6–10, respective elution fractions 1–5.